Sia Analiz
Sensitization
Sensitization studies are performed to determine whether the test substance has the potential to cause adverse local or systemic allergy and/or sensitization after repeated or prolonged exposure. Different in vivo tests are available for this purpose: Guinea Pig Maximization Test (Magnusson-Kligman Method), Murine Local Lymph Node Test (LLNA), Buehler Test.
The OECD 442B test is used to analyse immunological reactions in order to assess skin sensitisation. ELISA (Enzyme-Linked Immunosorbent Assay) testing plays a critical role in this process. Our facility conducts ELISA tests using the latest technologies to ensure high sensitivity and accuracy. These tests are used to detect specific antibody-antigen interactions, particularly in lymph nodes. The analyses we perform under the OECD 442B test are supported by the reliable data provided by ELISA tests. This ensures that even low concentrations of substances in biological samples can be accurately analysed, providing reliable and comprehensive results. Our ELISA tests meet the highest standards for immunoassay quality control.
Guinea Pig Maximization Test is frequently performed by expert and experienced personnel in SIA Analysis Laboratories with GLP declaration of conformity. If there is a specific request, LLNA or Buehler tests are also performed. Sensitization tests are carried out in accordance with national/international standards within the scope of OECD 406, OECD 442, EPA 870.2600 and ISO 10993-10.